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nanoFlu-Chl probe (chlorophyll-a)

monitoring the proliferation of algae

Sonde cyanobactéries, chlorophylle-a

Algae can be represented under various shapes, like the green, brown, red algae and micro-algae. They grow in water and more specifically in sunny environments, because they produce their energy from light thanks to photosynthesis. Algae can then proliferate suddenly and induce either health risks if they release toxins and risks for the local fauna and flora. On the other hand, algae can be used for diverse application in biotechnologies and especially for their biomass valorization and energy production.

The nanoFlu-Chl probe is a submersible miniaturized fluorimeter for the fluorescence measurement of chlorophyll-a, found in algae and cyanobacteria. The light emitted by the probe (470 nm) make fluoresce the chlorophyll flowing in front of the optical window. This visible light emitted by the fluorescence, of a higher wavelength (685 nm), is then detected and measured by a photodiode integrated in the probe.

The probe works without sampling and does not require calibration. The only maintenance operation consists in recalibrate the system once every 2 years.The sensor has many accessories to allow its integration in the process or the natural environment, to follow the variations of water levels or to automate its cleaning.

 

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Measurement principle

Fluorescence :

A specific LED emits a stimulation light at 470 nm, guided to form a light cone of about ten centimeters in front of the probe. The chlorophyll-a contained in the phytoplancton pass through this fluorescent stimulation cone and return a longer wavelength light at 685 nm.
This detection light, relative to the phycocyanin concentration of the water, is then measured by a photodiode. Increasing the amount of phycocyanin is a relevant indicator of the development of cyanobacteria in water.

Applications

  • Recreational water, lake and river monitoring
  • Drinking water resource monitoring
  • Measurements and regulations in algocutlure (phycoculture)
  • Environmental monitoring instrument

Advantages

  • In situ measurements, no sampling or reagents
  • Instant measurement
  • Optical window with coating to minimize fouling
  • Portable use possible
  • Automatic compensation of LED power loss and temperature
Measurement principlefluorescence
ultra luminous Blue LED
Detectorphotodiode with interferential filter
Parameterchlorophyll-a
Measurement range0...200 µg/l chlorophyll-a
Accuracy± 5%
Auto controlautomatic compensation of light source intensity variations due to wear and temperatures
Interferenceautomatic removal of ambient light
Response timeT100 : 6 s
Measurement interval3 s
Probe Body Materialsstainless steel (1.4571 / 1.4404) or titanium (3.7035)
Dimensions (L x Ø)171 mm x 36 mm
Weight0.5 kg stainless steel - 0.4 kg titanium
InterfaceEthernet (TCP/IP)
RS-232 ou RS-485 (Modbus RTU)
Power supply and consumption12 ... 24 VDC, <1 W (<1.6 W with network)
Maintenance≦ 0.5 h / month (cleaning of the optical window)
Calibration interval24 months
Maximum operating pressure- with SubConn waterproof connector: 30 bar
- with fixed cable: 3 bars
- in flow cell: 1 bar, 2 ... 4 L / min
ProtectionIP68
Temperature of the field or sample+2 ... 40 °C
Inflow velocity0,1 ... 10 m/s