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nanoFlu-Blue probe (cyanobacteria)

Cyanobacteria monitoring probe

Sonde cyanobactéries, chlorophylle-a

Cyanobacteria are micro-organism also called micro-algae or blue algae. They multiply in water and more particularly in lakes and rivers. Cyanobacterial bloom phenomenon has to be monitored, because the fast growth of these organism can induce the release of toxins such as microcystin in recreational water and drinking water resources, establishing an important health risk. It is also necessary to control cyanobacteria in aquaculture, where their development can jeopardize fish farms. Moreover, these organisms are the target of many research in biotechnologies, both for their biomass valorization and the production of interesting metabolites such as spirulin, or even for energy production.

The nanoFlu-Blue probe is a submersible miniaturized fluorimeter for the fluorescence measurement of phycocyanin, a pigment found in cyanobacteria. The light emitted by the probe (620 nm) make fluoresce this colored pigment flowing in front of the optical window. This visible light emitted by the fluorescence, of a higher wavelength (655 nm), is then detected and measured by a photodiode integrated in the probe.

The probe works without sampling and does not require calibration. The only maintenance operation consists in recalibrate the system once every 2 years.The sensor has many accessories to allow its integration in the process or the natural environment, to follow the variations of water levels or to automate its cleaning.

 

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Measurement principle

Fluorescence :

A specific LED emits a stimulation light at 620 nm, guided to form a light cone of about ten centimeters in front of the probe. The phycocyanin pigments contained in the cyanobacteria pass through this fluorescent stimulation cone and return a longer wavelength light at 655 nm.
This detection light, relative to the phycocyanin concentration of the water, is then measured by a photodiode. Increasing the amount of phycocyanin is a relevant indicator of the development of cyanobacteria in water.

Applications

  • Recreational water, lake and river monitoring
  • Drinking water resource monitoring
  • Aquaculture monitoring
  • Laboratory measurement

Advantages

  • In situ measurements, no sampling or reagents
  • Instant measurement
  • Optical window with coating to minimize fouling
  • Portable use possible
  • Automatic compensation of LED power loss and temperature
Measurement principlefluorescence
ultra luminous Blue LED
Detectorphotodiode with interferential filter
Parameterphycocyanin
Measurement range0...200 µg/l phycocyanin
Accuracy± 5%
Auto controlautomatic compensation of light source intensity variations due to wear and temperatures
Interferenceautomatic removal of ambient light
Response timeT100 : 6 s
Measurement interval3 s
Probe Body Materialsstainless steel (1.4571 / 1.4404) or titanium (3.7035)
Dimensions (L x Ø)171 mm x 36 mm
Weight0.5 kg stainless steel - 0.4 kg titanium
InterfaceEthernet (TCP/IP)
RS-232 ou RS-485 (Modbus RTU)
Power supply and consumption12 ... 24 VDC, <1 W (<1.6 W with network)
Maintenance≦ 0.5 h / month (cleaning of the optical window)
Calibration interval24 months
Maximum operating pressure- with SubConn waterproof connector: 30 bar
- with fixed cable: 3 bars
- in flow cell: 1 bar, 2 ... 4 L / min
ProtectionIP68
Temperature of the field or sample+2 ... 40 °C
Inflow velocity0,1 ... 10 m/s